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KMID : 0368820050440040497
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Journal of the Korean Neuropsychiatr Association
2005 Volume.44 No. 4 p.497 ~ p.504
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Cell Viability and Proliferation Activity of Peripheral Lymphocytes in Patients with Alzheimer’s Disease.
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Lee Su-Zie
An Sang-Mee
Kim Doh-Kwan
Kwon Young-Ah
Kim Hye-Ran
Chung Sung-Ho
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Abstract
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Objectives: There are evidences of apoptotic neuronal cell death in Alzheimer’s disease (AD). Recent studies suggested AD pathogenesis in the central nervous system as well as in peripheral lymphocytes. The object of this study is to compare the cell viability and the proliferation activity in AD patients with healthy normal control by using peripheral lymphocytes.
Methods: We analyzed the cell viability and the proliferation activity of phytohemagglutinin (PHA)-activated lymphocytes from 73 AD patients and 31 normal contols. The cell viability and the proliferation activity were measured at baseline (T0), 24 hours (T24), 48 hours (T48), 72 hours (T72), 96 hours (T96), by the tryphan blue method and the BrdU proliferation activity method, respectively.
Results: The cell viability of PHA-activated peripheral lymphocytes in AD patients was significantly decreased at T72, T96 compared with healthy controls (F=8.034, p<0.001). In AD patients, the decline of proliferation activity appeared in earlier than healthy normal controls.
Conclusion: This study suggests that there is a decreased cell viability and the proliferation activity of peripheral lymphocytes in AD patients. These finding may be related with the increased apoptosis in Alzheimer’s disease.
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KEYWORD
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Alzheimer’s disease, Cell viability, Proliferation activity, Peripheral lymphocytes
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